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Bacteria Test Kit


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Bacteria Test Kit

The cleanest, best tasting water may contain undetected contaminants. Water contaminated with harmful bacteria, nitrates or nitrites can cause diarrhea, cramps, nausea, headache, serious illness, even death. Accurately detect the presence of  coliform bacteria, including E. coli. in your drinking water by using a Bacteria Test Kit.


Bacteria in Drinking Water

Can bacteria in water make me sick?

The transmission of disease through drinking water is one of the primary concerns for a safe water supply. Human illnesses such as typhoid, dysentery, chlorea, hepatitis, and giardiasis have been linked to drinking water contaminated by human waste.

Can bacteria in water affect livestock?

Bacteria levels for livestock vary with intended water use (Table 1). Adult animals are more tolerant of bacteria than young animals. Water for cleaning and sanitizing must be of very high quality to prevent infections and contamination of food products.

Table 1. Bacteria guidelines for livestock water supplies
Adult animals 1000 fecal coliforms/100 ml
Young animals 1 fecal coliform/100 ml
Dairy wash water 1 coliform/100 ml

How to tell if water is contaminated with bacteria.

Testing a water supply for a specific disease causing organisms is expensive. Handling and culturing disease organisms requires special training and equipment. Also, if the water supply is being contaminated by human wastes, but the disease organism is not present the day a sample is taken, the risk of future exposure to the illness is still present.

Instead, water supplies are tested for an indicator of human or animal waste-coliform bacteria. Coliforms do not cause disease. They are, however, always present in the digestive systems of humans and animals and can be found in their wastes. Coliforms are also present in the soil and plant material.

If a water supply is found to contain coliform bacteria it may be contaminated by sewage or manure, and there is a risk of exposure to water-borne disease. The test for coliform bacteria is relatively inexpensive (usually $6 to $15 per sample) and easy to perform.

To determine whether the bacteria present is from human or animal waste, additional tests must be performed. Coliform bacteria also could come from natural sources such as soil or decaying vegetation. Some coliform bacteria are only present in fecal material; these are called fecal coliforms. These bacteria indicate the presence of human or animal waste.

A test for a third bacteria, fecal streptococci, must be performed to determine if it is of human or animal origin. The ratio of fecal coliforms to fecal streptococci vary for different animals (Table 2). If the ratio is near four, the waste is from humans. The ratio is less than one for animal wastes.

Another type of bacteria, referred to as iron bacteria, is a major nuisance in many well water supplies. (Iron bacteria should not to be confused with iron dissolved in water that causes red water and stains on clothing and plumbing fixtures.) This naturallyoccurring bacteria does not cause disease, but does form a reddishbrown slime that coats the inside of pipes, fouls pumps and clogs waterers.

Table 2. Typical fecal coliform/fecal streptococci ratios for humans and various animals
Human 4.4
Duck 0.6
Sheep 0.4
Chicken 0.4
Pig 0.4
Cow 0.2
Turkey 0.1

How to collect and handle a water sample

Proper collection and handling of a water sample is critical for a meaningful water test. Sample containers should always be obtained from the testing laboratory because containers may be specially prepared for a specific contaminant. Sampling and handling procedures will depend on the specific water quality concern and should be followed carefully. If the water is being treated, it may be necessary to sample both before and after the water goes through the treatment equipment.

Bacteria sampling

Water samples for bacteria tests must always be collected in a sterile container. Take the sample from an inside faucet with the aerator removed. Sterilize by flaming the end of the tap with a disposable butane lighter. Run the water for five minutes to clear the water lines and bring in fresh water. Do not touch or contaminate the inside of the bottle or cap. Carefully open the sample container and hold the outside of the cap. Fill the container and replace the top.

Refrigerate the sample and transport it to the testing laboratory within six hours (in an ice chest). Many labs will not accept bacteria samples on Friday so check the lab's schedule. Mailing bacteria samples is not recommended because laboratory analysis results are not as reliable.

Iron bacteria forms a very obvious slime on the inside of pipes and fixtures. A water test is not needed for identification. Check for a reddish-brown slime inside a toilet tank or where water stands for several days.

What should I do if my water is contaminated with bacteria?

First, don't panic' Bacterial contamination is very common. Studies have found that over 40 percent of private water supplies are contaminated with coliform bacteria. Spring water supplies are the most frequently contaminated, with over 70 percent containing coliform bacteria.

Improving protection of a well or spring from the inflow of surface water is an important option to consider if the supply is contaminated with bacteria. It is important to remember that the groundwater is not necessarily contaminated in these cases, rather the well is acting to funnel contaminants down into the groundwater.

Although well pits were the common method of construction several years ago, they are no longer considered sanitary construction.

A properly protected well is evidenced by the well casing extending above the surface of the ground and the ground sloping away from the well to prevent water from collecting around the casing.

A properly protected spring is developed underground and the water channeled to a sealed spring box. At no time should the water be exposed to the ground surface.

Keeping the plumbing system clean is an important part of maintaining a sanitary water supply. Anytime work is performed on the plumbing or pump, the entire water system should be disinfected with chlorine. Simply pulling the pump out of the well, setting it on the grass to work on it, and returning it to the well is enough to contaminate the well with bacteria.

The procedure for cleaning and sanitizing a well or spring with chlorine is called shock chlorination. Concentrations of chlorine ranging from 50 to 200 mg/1 are used in the shock chlorination process. This is 100 to 400 times the amount of chlorine found in "city water." The highly chlorinated water is held in the pipes for 12 to 24 hours before it is flushed out and the system is ready to be used again.

Periodic shock chlorination also may be effective in reducing an iron bacteria problem. The amount of chlorine needed to shock chlorinate a water system is determined by the amount of water standing in the well.

Table 3 lists the amount of chlorine laundry bleach or powdered high-test hypochlorite (HTH) that is needed for wells. If in doubt, it is better to use more chlorine than less.

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Reference:
Karen M. Mancl. Water Quality Specialist & Agricultural Engineering. Bacteria in Drinking Water. Bulletin 795. The Ohio State University. July 5, 2004 <http://ohioline.osu.edu/b795/b795_1.html; http://ohioline.osu.edu/b795/b795_2.html;  http://ohioline.osu.edu/b795/b795_3.html>.

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Last Updated: Friday, December 14, 2007